Anal Cytology

The “Anal Pap” is a screening used in high risk population to identify pre-malignant and malignant cytologic changes of the anal epithelium.

Indications:

Detection and characterization of pre-malignant and malignant changes.

Specimen Required:

Sample collected from anal canal including squamocolumnar junction submitted in a SurePath liquid based cytology vial.

Supplies:

SurePath vial, break off spatula and cytobrush.

Collection Procedure:

Label SurePath vial with appropriate patient name and MRN that corresponds to the information provided on the requisition. Insert the cytobrush 1.5 cms into the anal canal. Slowly retract the cytobrush with a twisting motion. Break off the cytobrush tip and drop in vial. Use the spatula to perform a sweep of the anus. Break off the spatula tip and drop in vial. Send labeled vial with collection device tips submitted in vial and Cytology Requisition to lab for processing.

HPV testing on anal specimens is a referred test. A separate specimen must be submitted. Specimens for HPV testing on anal samples must be collected using a Digene Cervical Sampler/Specimen Transport. Refer to ANAL HPV HIGH RISK in the Test Catalog.

Adopted MKurtinecz,          2/05
Reviewed MKurtinecz, TSp 2/05
Reviewed MKurtinecz, TSp 1/06
Reviewed MKurtinecz,TSp  5/07
Revised MKurtinecz, TSp    5/05
Reviewed: MKurtinecz, TSp 7/08
Reviewed: MKurtinecz, TSp 8/09