Urine is commonly evaluated cytologically
for the presence of malignant cells in the detection of urologic malignancies.
Method of specimen collection as well as time of collection will affect
the cytologic evaluation in many instances.
| Indications: |
Detection
and characterization of malignant cells and other urologic abnormalities
in symptomatic (usually hematuria) patients; screening for malignancy
in selected individuals at high risk for the development of urologic
malignancy. |
| Specimen Required: |
50 mL of
an appropriately collected voided, catheterized, or cystoscopically
obtained urine specimen. |
| Supplies: |
Clean collection
container of appropriate size. Fixative (Saccomanno Fixative). |
| Collection
Procedure: |
For purposes
of obtaining the greatest yield of diagnostic material, a second
morning voided urine specimen should be obtained, if possible.
A midstream, clean catch specimen is recommended to avoid vaginal
contamination in female patients. A midstream specimen, not necessarily
clean catch, is recommended for male patients. If the patient
must be catheterized to obtain the specimen, this should be noted
on the specimen requisition as catheterization can lead to artifacts
which may be misinterpreted without the knowledge that the specimen
was catheterized. Submit the specimen to the Cytopathology Laboratory
along with the completed cytology request form. Add an equal
volume of Saccomanno fixative (if sample size is too large to
accommodate this volume, a well-mixed aliquot of the specimen
with an equal volume of fixative may be utilized. With
Saccomanno fixative added, specimen may be transported at room
temperature. If fixative is not available, the specimen
should be refrigerated or kept on wet ice until transported to
the lab. |
| Indications: |
Detection
of suspected malignancy utilizing lavage specimens obtained cystoscopically
(bladder washing, ureteral washing); staging of urologic malignancies. |
| Specimen
Required: |
10 mL (or
more) of an appropriately collected cystoscopically derived specimen. |
| Supplies: |
Standard
cystoscopy equipment. Clean collection container of appropriate
size. Fixative (Saccomanno Fixative). |
| Collection
Procedure: |
Using standard
cystoscopy technique, obtain washing specimens, carefully denoting
specific specimen sites for each specimen on the requisition.
Add an equal volume of Saccomanno fixative to the specimen
Submit the specimen to the Cytopathology Laboratory along with
the completed
cytology request form. With Saccomanno fixative added,
specimen may be transported at room temperature If fixative
is not available, the specimen should be refrigerated or kept
on
wet ice until transported to the lab.
|
Urine for BK Virus |
| Indications: |
Detection and characterization of BK viral changes
in urologic specimens (voided urines) and potentially other urologic
abnormalities in transplant patients. |
| Specimen Required: |
50 mL of an appropriately collected voided
urine specimen. |
| Supplies: |
Clean collection container of appropriate size. Fixative
(Saccomanno Fixative). |
| Collection Procedure: |
For purposes of obtaining the greatest yield of diagnostic
material, a second morning voided urine specimen should be obtained,
if possible. A midstream, clean catch specimen is recommended to
avoid vaginal contamination in female patients. A midstream specimen,
clean catch, is recommended for male patients. If the patient must
be catheterized to obtain the specimen, this must be noted on the
specimen requisition. Catheterization can cause cellular artifacts
which may be misinterpreted without the knowledge that the specimen
was catheterized. Submit the specimen to the Cytopathology Laboratory
along with the completed Cytology Request form stating Urine for
BK Virus. Add an equal volume of Saccomanno fixative (if sample size
is too large to accommodate this volume, a well-mixed aliquot of
the specimen with an equal volume of fixative may be utilized. With
Saccomanno fixative added, specimen may be transported at room temperature.
If fixative is not available, the specimen should be refrigerated
or kept on wet ice until it is transported to the lab. |
Urine for Hemosiderin (Iron Stain) |
| Indications: |
Detection and characterization of hemosiderin-laden
cslls in urologic specimens and potentially other urologic abnormalities. |
| Specimen Required: |
50 mL of an appropriately collected voided, catheterized,
or cystocopically obtained urine specimen. |
| Supplies: |
Clean collection container of appropriate size. |
| Collection Procedure: |
For purposes of obtaining the greatest yield of diagnostic
material, a second morning voided urine specimen should be obtained,
if possible. A midstream, clean catch specimen is recommended to
avoid vaginal contamination in female patients. A midstream specimen,
clean catch, is recommended for male patients. If the patient must
be catheterized to obtain the specimen, this must be noted on the
specimen requisition. Catheterization can cause cellular artifacts
which may be misinterpreted without the knowledge that the specimen
was catheterized. Submit the specimen to the Cytopathology Laboratory
along with the completed Cytology Request form stating request for
hemosiderin. The specimen should be refrigerated or kept on wet ice
until it is transported to the lab. |
UroVysion™ for voided urine specimens |
| Indications: |
The UroVysion TM assay is a FISH assay for the detection of urothelial carcinoma. The UroVysion TM probe set contains probes to the centromeres of chromosomes 3, 7, and 17. And a locus specific probe for 9p21 band. |
| Specimen Required: |
Minimum >33ml of voided urine with 17ml of appropriate preservative added (total volume 50ml). The ratio of voided urine to preservative should be approximately 2:1 if larger volume of voided urine is collected. Multiple specimen containers may be used. |
| Supplies: |
Clean collection container of appropriate size. Saccomanno Preservative |
| Collection Procedure: |
For purposes of obtaining the greatest yield of diagnostic material, a second morning voided urine specimen should be obtained, if possible.
A midstream, clean catch specimen is recommended to avoid vaginal contamination in female patients.
A midstream specimen, not necessarily clean catch, is recommended for male patients.
Submit the specimen to the Cytopathology Laboratory along with the completed cytology request form and note date/time of collection. Add Saccomanno preservative.
If necessary, may send 2 specimen containers, label both containers as appropriate.
With Saccomanno preservative added, specimen may be transported at room temperature. If transport time exceeds 12 hours or temperature exceeds 25°C, refrigerate specimen and send with ice packs. If fixative is not available, the specimen must be refrigerated and kept on ice packets during transport to the lab. |
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Reviewed/revised: 07/20/2011
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