Skip to main content

Specimen Collection Manual and Test Catalog

  or
  or
  or

T4 T8 LYMPHOCYTE SUBSET PANEL

Geisinger Epic Procedure Code:  LAB3127    Geisinger Epic ID:  15201

SPECIMEN COLLECTION
Specimen type: 

Whole blood


Preferred collection container: 
Specimen required: 

Two (2) properly drawn K2 EDTA tubes whole blood.


Special notes: 

Collect TWO (2) tubes. Do not combine tubes.



SPECIMEN PROCESSING
Processing instructions: 

Do not centrifuge, refrigerate or freeze.


Transport temperature: 

Room temperature. Do not expose to extremes of temperature.


Specimen stability: 

Lavender-top (EDTA) tube stable for 48 hours at room temperature.


Rejection criteria: 

Specimen stability exceeded. Clotted specimen. Hemolyzed specimen.



TEST DETAILS
Reference interval: 
AGE:CD3 %CD3+
CD4%
CD3+
CD8%
CD19 %CD56+
CD16 %
CD4:CD8 RATIO
17- adult61-8835-6213-354.0-213.0-210.8-3.5
1052-7825-489.0-35.08.0-24.06.0-27.0.9-3.4
555-7827-5319.0-34.010.0-31.04.0-26.00.9-2.6
243-7623-4814.0-33.014.0-44.04.0-23.00.9-2.9
15 M39-7325-5011.0-32.017.0-41.03.0-16.00.9-3.7
9 M54-7631-5412.0-28.015.0-39.03.0-17.01.3-3.9
5 M50-7733-5813.0-26.013.0-35.02.0-13.01.6-3.8
2 M48-7533-5811.0-25.014.0-39.02.0-14.01.7-3.9
7 D60-8541-689.0-23.04.0-26.03.0-23.01.3-6.3
028-7617-5210.0-41.05.0-22.06.0-58.01.0-2.6


 

AGE:CD3 AbsoluteCD3+/CD4+ AbsoluteCD3+/CD8+ AbsoluteCD19/CD3-
Absolute
CD56+CD16 Absolute
17 - adult510-2265330-1520150-79033-55050-400
10800-3500400-2100200-1200200-60070-1200
5700-4200300-2000300-1800200-160090-900
2900-4500500-2400300-1600200-2100100-1000
15 M1400-8000900-5500400-2300600-3100100-1400
9 M1600-67001000-4600400-2100600-2700200-1200
5 M2400-69001400-5100600-2200700-2500100-1000
2 M2300-65001500-5000500-1600600-3000100-1300
7 D2300-70001700-5300400-170060-1900200-1400
0600-5000400-3500200-190040-1100100-1900


*Adult ranges established by normal study performed at GMC 2002
*Pediatric ranges from : The Journal of Pediatrics Vol. 130(3), March 1997, pp 388-393


Additional information: 

Analysis performed according to CDC Guidelines.


CPT code(s):  86355, 86357, 86359, 86360
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Total T Cells, T-helper cells, T- suppressor cells, B cells and NK (natural killer) cells.


Methodology: 
Flow Cytometry
Synonyms: 

CDC Lymphocyte panel, Immunodeficiency profile, T and B cell subsets, CD3, CD4, CD8, CD19, CD56, T4T8, CD4CD8 Lymphocyte Panel


Clinical significance: 

Lymphocyte subset anlysis is useful for evaluation of immunocompetency in suspected cases of congenital or acquired immunodeficiency.


Doctoral Director(s): 
Kai Zhang M.D.
Review Date:  10/04/2024

Performing Locations