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Specimen Collection Manual and Test Catalog

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CD4P T-CELL SUBSET PANEL

Geisinger Epic Procedure Code:  LAB1968    Geisinger Epic ID:  14856

SPECIMEN COLLECTION
Specimen type: 

Whole blood


Preferred collection container: 
Specimen required: 

Two (2) properly drawn K2 EDTA tubes whole blood


Special notes: 

Collect TWO tubes. Do not combine tubes.



SPECIMEN PROCESSING
Processing instructions: 

Do not centrifuge, refrigerate or freeze.


Transport temperature: 

Room temperature. Do not expose to extremes of temperature.


Specimen stability: 

Lavender (EDTA) tube stable for 48 hours at room temperature.


Rejection criteria: 

Stability exceeded, hemolyzed or clotted.



TEST DETAILS
Reference interval: 
AGE:CD3 %CD3+/CD4+%CD3+/CD8+ %CD4:CD8 RATIO
17- adult61 - 8835 - 6213 - 350.8 - 3.5
1052-7825-489.0-35.00.9-3.4
555-7827-5319.0-34.00.9-2.6
243-7623-4814.0-33.00.9-2.9
15 M39-7325-5011.0-32.00.9-3.7
9 M54-7631-5412.0-28.01.3-3.9
5 M50-7733-5813.0-26.01.6-3.8
2 M48-7533-5811.0-25.01.7-3.9
7 D60-8541-689.0-23.01.3-6.3
028-7617-5210.0-41.01.0-2.6



 

AGE:CD3 AbsoluteCD3+/CD4+ AbsoluteCD3+/CD8+ Absolute
17- adult510-2265330-1520150-790
10800-3500400-2100200-1200
5700-4200300-2000300-1800
2900-4500500-2400300-1600
15 M1400-8000900-5500400-2300
9 M1600-67001000-4600400-2100
5 M2400-69001400-5100600-2200
2 M2300-65001500-5000500-1600
7 D2300-70001700-5300400-1700
0600-5000400-3500200-1900


*Adult ranges established by normal study performed at GMC 2002
*Pediatric ranges from : The Journal of Pediatrics Vol. 130(3), March 1997, pp 388-393


Additional information: 

Analysis performed according to CDC Guidelines.


CPT code(s):  86360, 86359
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Total T-cells (CD3);T-helper (CD4+) cells and T-suppressor (CD8+) by % of white blood cells and absolute count with a CD4:CD8 ratio.


Methodology: 
Flow Cytometry
Synonyms: 

T4 Count, CD4 Count, Absolute CD4, CD4P


Clinical significance: 

Evaluation of T-cell markers is useful in evaluating immune status in immunodeficiency diseases.


Doctoral Director(s): 
Kai Zhang M.D.
Review Date:  10/04/2024

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