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Specimen Collection Manual and Test Catalog

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FECAL OCCULT BLOOD, EIA

Geisinger Epic Procedure Code:  LAB2269    Geisinger Epic ID:  29481

SPECIMEN COLLECTION
Specimen type: 

Random stool


Preferred collection container: 
Specimen required: 

Inpatient / ED: Non-sterile specimen container 
Off-site specimen: FOBT Collection Vial


Special notes: 

Offsite specimens: Do not overfill collection vial. All fields (Collection Date, Name, MRN, or DOB if MRN is not available) in the label must be filled out. Patient Instructions 
Inpatient / ED: Testing limited to pediatric patients only. Collect feces in a clean leak-proof container. Do not add fixative or preservative.


Patient preparation: 

Patients with hemorrhoids or menstrual bleeding should wait until bleeding ceases.



SPECIMEN PROCESSING
Transport temperature: 

Room temperature (preferred) or refrigerated.


Specimen stability: 

Collection vial: Room temperature: 6 days. Refrigerated: 30 days. 
Non-sterile containers: 24 hours.


Rejection criteria: 

Overfilled specimens: samples that are not liquid enough to place drops on the cartridge. Specimen that does not have fecal matter added to the collection vial and the liquid in the vial is clear. Specimen past the stability limits.



TEST DETAILS
Reference interval: 

Negative.


Interpretation guide: 

Positive test indicates the presence of human hemoglobin (blood) in stool. May be upper GI or lower GI.


CPT code(s):  82274
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Immunoassay for human hemoglobin.


Methodology: 
Immunoassay
Synonyms: 

Hemosure iFOBT, Fecal Occult Blood Test, Occult blood test, FIT, iFOBT, FOBT, Immunological FOBT, Occult Blood Screen (EIA Mail in)


Clinical significance: 

This test detects the presence of human hemoglobin in stool that may be of gastrointestinal or colorectal origin. It is used as a screen to detect polyps and lesions before they become cancerous.


Doctoral Director(s): 
Hoi-Ying Elsie Yu, PhD, DABCC, FADLM
Sheng-Ying (Margaret) Lo, PhD, DABCC
Benjamin Andress, PhD, DABCC
Review Date:  01/14/2025

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