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Specimen Collection Manual and Test Catalog

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25-HYDROXY VITAMIN D

Geisinger Epic Procedure Code:  LAB1489    Geisinger Epic ID:  33530

SPECIMEN COLLECTION
Specimen type: 

Serum (preferred) or plasma


Preferred collection container: 
Alternate collection container: 
3 mL green/yellow-top (plasma separator) tube
Specimen required: 

0.5 mL serum (preferred) or plasma ; minimum 0.3 mL.



SPECIMEN PROCESSING
Processing instructions: 

Allow serum to clot. Centrifuge to separate serum or plasma within 2 hours of collection.


Transport temperature: 

Refrigerated.


Specimen stability: 

Room temperature: 8 hours. Refrigerated: 4 days. Frozen: 24 weeks.


Rejection criteria: 

Stability limits exceeded.



TEST DETAILS
Interpretation guide: 

Deficient <20 ng/mL 
Insufficient 20 - 29 ng/mL
Recommended/Optimum 30 - 50 ng/mL 
Vitamin D intoxication is rare. If suspicious of Vitamin D toxicity, evaluation of serum calcium and PTH is recommended.


CPT code(s):  82306
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Total vitamin 25-OH D. Vitamin D2, 25-OH, Vitamin D3, 25-OH.


Methodology: 
Electrochemiluminescence
Synonyms: 

Total Vitamin D; 25-OH Vitamin D, 25-OH D, 25OHVD


Clinical significance: 

Vitamin D3 and Vitamin D2 are the most abundant forms of vitamin D in the body. Vitamin D3 is synthesized in the skin while D2 is derived from nutritional sources. D3 and D2 are converted to 25OH vitamin D in the liver. 25OH Vitamin D is the best indicator of vitamin D nutritional status.


Doctoral Director(s): 
Hoi-Ying Elsie Yu, PhD, DABCC, FADLM
Sheng-Ying (Margaret) Lo, PhD, DABCC
Review Date:  12/27/2024

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