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Specimen Collection Manual and Test Catalog

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ANTINUCLEAR ANTIBODY (ANA), IFA

Geisinger Epic Procedure Code:  LAB1768    Geisinger Epic ID:  41286

SPECIMEN COLLECTION
Specimen type: 

Serum


Preferred collection container: 
Alternate collection container: 
6 mL red-top (plain, non-serum separator) tube
Specimen required: 

0.5 mL serum; minimum 0.2 mL



SPECIMEN PROCESSING
Processing instructions: 

Allow to clot. Centrifuge and submit 1 mL serum.


Transport temperature: 

Refrigerated (preferred). Room temperature (stable 8 hours) and frozen specimens also acceptable.


Specimen stability: 

Room temperature: 8 hours. Refrigerated 2-8°C: 4 days (96 hours). Frozen: 6 months.


Rejection criteria: 

Specimen stability exceeded; specimen grossly hemolyzed.



TEST DETAILS
Reference interval: 

<1:80


Additional information: 

This assay is intended for the Rheumatology Department for those patients who were ANA EIA negative.


CPT code(s):  86039
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

ANA titer and pattern.


Methodology: 
Indirect Immunofluorescent Antibody (IFA)
Synonyms: 

ANA IFA, ANA pattern and titer, centromere, ANA titer, Antinuclear AB titer and pattern, ANAIFA


Clinical significance: 

The presence of ANA antibodies in correlation with clinical findings and other laboratory tests can aid in the diagnosis of certain rheumatic diseases including systemic lupus erythematosus (SLE), Sjogren's syndrome, mixed connective tissue disease, scleroderma, drug-induced lupus, and rheumatoid arthritis.


Doctoral Director(s): 
Benjamin Andress, PhD, DABCC
Hoi-Ying Elsie Yu, PhD, DABCC, FADLM
Review Date:  11/25/2024

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