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Specimen Collection Manual and Test Catalog

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TESTOSTERONE: TOTAL, FREE AND BIOAVAILABLE

Geisinger Epic Procedure Code:  LAB3140    Geisinger Epic ID:  35861

SPECIMEN COLLECTION
Specimen type: 

Plasma or serum


Preferred collection container: 
Specimen required: 

2 mL serum or plasma; minimum 0.5 mL.


Patient preparation: 

For patients receiving high biotin doses, wait until 8 hours after last dose.



SPECIMEN PROCESSING
Processing instructions: 

Allow serum to clot. Centrifuge to separate serum or plasma within 2 hours of collection.


Transport temperature: 

Refrigerated (preferred) or frozen.


Specimen stability: 

Room temperature: 5 days. Refrigerated: 1 week. Frozen: 4 months.


Rejection criteria: 

Stability limits exceeded.



TEST DETAILS
Reference interval: 

Sex hormone binding globulin (SHBG): 
Males: 12-91 nmol/L 
Females: 17-176 nmol/L

Total Testosterone: 
Males: 18-49 years 249-836 ng/dL
>49 years 193-740 ng/dL
Females: > 18 years 2-48 ng/dL 

Albumin: 3.8-5.0 g/dL

Calculated free testosterone: 
Males: 35-130 pg/mL
Females: 0.2-7.8 pg/mL

Calculated bioavailable testosterone: 
Males: 79-335 ng/dL
Females: 0.4-19 ng/dL


CPT code(s):  82040, 84403, 84270
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Sex hormone binding globulin (SHBG), total testosterone, albumin, calculated free testosterone, calculated bioavailable testosterone.


Methodology: 
Electrochemiluminescence
Spectrophotometric (Colorimetric)
Synonyms: 

TESTOB


Doctoral Director(s): 
Hoi-Ying Elsie Yu, PhD, DABCC, FADLM
Sheng-Ying (Margaret) Lo, PhD, DABCC
Review Date:  12/27/2024

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