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Specimen Collection Manual and Test Catalog

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DOUBLE STRANDED DNA ANTIBODY, INDIRECT IMMUNOFLUORESCENCE ASSAY

Geisinger Epic Procedure Code:  LAB2195    Geisinger Epic ID:  41802
Test Restriction:  ORDER LIMITED TO NEUROLOGY, RHEUMATOLOGY, NEPHROLOGY

SPECIMEN COLLECTION
Specimen type: 

Serum


Preferred collection container: 
Alternate collection container: 
6 mL red-top (plain, non-serum separator) tube
Specimen required: 

0.5 mL serum; minimum 0.2 mL



SPECIMEN PROCESSING
Processing instructions: 

Allow to clot. Centrifuge and submit 0.5 mL serum.


Transport temperature: 

Refrigerated (preferred). Room temperature (stable 8 hours) or frozen specimen also acceptable.


Specimen stability: 

Room temperature: 8 hours. 2-8°C: 72 hours. Frozen: 6 months.


Rejection criteria: 

Specimen stability exceeded; specimen grossly hemolyzed.



TEST DETAILS
Reference interval: 

<1:10


CPT code(s):  86225
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Antibody detection and titer if positive.


Methodology: 
Indirect Immunofluorescent Antibody (IFA)
Synonyms: 

Anti-DNA Antibody, Anti-Native DNA, Anti-Double Stranded DNA, Crithidia Assay, Double stranded anti-DNA antibody, DSDNA


Clinical significance: 

High titers of antibodies to double-stranded DNA (dsDNA) are associated with systemic lupus erythematosus (SLE). The titer may decrease with succesful therapy and increase in acute recurrence of the disease.


Doctoral Director(s): 
Benjamin Andress, PhD, DABCC
Hoi-Ying Elsie Yu, PhD, DABCC, FADLM
Review Date:  11/25/2024

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