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Specimen Collection Manual and Test Catalog

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1,25-DIHYDROXY VITAMIN D

Geisinger Epic Procedure Code:  LAB1476    Geisinger Epic ID:  43051

SPECIMEN COLLECTION
Specimen type: 

Serum


Preferred collection container: 
Alternate collection container: 
6 mL red-top (plain, non-serum separator) tube
Specimen required: 

1.0 ml (minimum 0.5 ml) in a plastic screw-capped vial.


Special notes: 

Note: If sample is submitted with less than 1.1 mL and needs to be repeated the sample will be cancelled with the comment "TNP-Initial testing necessitated a repeat, but there was insufficient sample to perform repeat".



SPECIMEN PROCESSING
Transport temperature: 

Room temperature.


Specimen stability: 

Room temperature: 28 days. Refrigerated: 28 days. Frozen: 28 days


Rejection criteria: 

Grossly hemolyzed, lipemic, or icteric specimens, or plasma samples are unacceptable for this test.



TEST DETAILS
CPT code(s):  82652
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Vitamin D,1,25 (OH)2,Total; Vitamin D3, 1,25 (OH)2; Vitamin D2, 1,25 (OH)2


Methodology: 
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Synonyms: 

Vitamin D,1,25 (OH)2,Total; Vitamin D3, 1,25 (OH)2; Vitamin D2, 1,25 (OH)2, Quest test code 16558, 125VD


Clinical significance: 

This test is used to measure the bio-active form of Vitamin D. This test is also used in the differential diagnosis of hypocalcemia and to monitor patients with renal osteodystrophy or chronic renal failure,


Review Date:  12/04/2024

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