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Specimen Collection Manual and Test Catalog

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1,25-DIHYDROXY VITAMIN D

Geisinger Epic Procedure Code:  LAB1476    Geisinger Epic ID:  43051

SPECIMEN COLLECTION
Specimen type: 

Serum


Preferred collection container: 
Alternate collection container: 
6 mL red-top (plain, non-serum separator) tube
Specimen required: 

0.6 ml (minimum 0.25 ml) in a plastic screw-capped vial.



SPECIMEN PROCESSING
Transport temperature: 

Room temperature.


Specimen stability: 

Room temperature: 28 days. Refrigerated: 28 days. Frozen: 28 days


Rejection criteria: 

Grossly hemolyzed, lipemic, or icteric specimens, or plasma samples are unacceptable for this test.



TEST DETAILS
CPT code(s):  82652
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Vitamin D,1,25 (OH)2,Total; Vitamin D3, 1,25 (OH)2; Vitamin D2, 1,25 (OH)2


Methodology: 
Chromatography/Mass Spectrometry
Synonyms: 

VIT D 1, 25,1,25 OH-D,1,25 Dihydroxycholecalciferol,1,25 Dihydroxyvitamin D,1,25 Vitamin D,Dihydroxycholecalciferol, Quest test code 16558, 125VD


Clinical significance: 

Calcitriol 1,25-Dihydroxyvitamin D - This test measures the bioactive form of vitamin D. It is used in the differential diagnosis of hypocalcemia and to monitor patients with renal osteodystrophy or chronic renal failure. This test is not suitable for diagnosis of vitamin D deficiency and monitoring supplementation in most patients. The 25-hydroxyvitamin D test is the recommended test for those purposes (N Engl J Med. 2007;357:266-281).


Doctoral Director(s): 
Randin Nelson MD
Review Date:  04/16/2026

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