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Specimen Collection Manual and Test Catalog

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ASPERGILLUS NIGER (M207) IGE

Geisinger Epic Procedure Code:  LAB1787    Geisinger Epic ID:  45689

SPECIMEN COLLECTION
Specimen type: 

Serum


Preferred collection container: 
Alternate collection container: 
3.5 mL gold-top (serum separator) tube
Specimen required: 

0.3 mL serum; minimum 0.15 mL



SPECIMEN PROCESSING
Transport temperature: 

Room temperature.


Specimen stability: 

Room temperature: 14 days. Refrigerated: 14 days. Frozen: 30 days.



TEST DETAILS
CPT code(s):  86003
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Methodology: 
Fluorescence Enzyme Immunoassay
Synonyms: 

Quest test code 349388, ASPERN


Clinical significance: 

This procedure is based on a fluorescent enzyme immunoassay (FEIA) technique. The allergen of interest, covalently coupled to an ImmunoCap, reacts with the specific IgE in the patients serum. After washing away nonspecific IgE, enzyme-labelled antibodies against IgE are added to form a complex. After incubation, unbound enzyme anti-IgE is washed away and the bound complex is then incubated with a developing agent. After the reaction is stopped, the fluorescence of the eluate is measured. The higher the fluorescence value, the more specific IgE is present in the specimen. To classify test results, fluorescence for patient samples is compared directly with fluorescence for a reference serum run in parallel. Results are reported in both kU/L and Conventional Class score.


Review Date:  12/04/2024

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