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Specimen Collection Manual and Test Catalog

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HIV ANTIGEN & ANTIBODY SCREEN W/ CONFIRMATION

Geisinger Epic Procedure Code:  LAB2467    Geisinger Epic ID:  45912

SPECIMEN COLLECTION
Specimen type: 

Serum


Preferred collection container: 
Specimen required: 

1 mL serum; minimum 1 mL



SPECIMEN PROCESSING
Processing instructions: 

Allow to clot. Centrifuge to separate serum within 2 hours of collection.


Transport temperature: 

Refrigerated (preferred).


Specimen stability: 

Room temperature: 7 days. Refrigerated(2 - 8°C): 4 weeks. Frozen: 3 months. No more than 5 freeze/thaw cycles.


Rejection criteria: 

Specimen will be rejected if stability limit is exceeded or if specimen is hemolyzed or icteric. Add-ons available only to unopened tube.



TEST DETAILS
Reference interval: 

Negative.


Interpretation guide: 

CDC Recommended Laboratory HIV Testing


CPT code(s):  87389
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Qualitative detection of antibodies to human immunodeficiency virus type 1 and type 2. Positive result will be reflexed to "HIV1/HIV2 antibody differentiation assay.


Methodology: 
Electrochemiluminescence Immunoassay
Synonyms: 

HIV-1/HIV-2 ANTIBODY;HIV antibody combo, HIV GEN 4, HIV4G


Clinical significance: 

HIV Ag/Ab Combo assay is an immunoassay for the simultaneous qualitative determination of human immunodeficiency virus (HIV) p24 antigen and antibodies to HIV type 1(HIV-1 group M and group O) and/or type 2 (HIV-2). Specimens with a reactive HIV AG/AB Combo assay result should be tested with a confirmatory assay that differentiates HIV-1 from HIV-2 antibodies.


Doctoral Director(s): 
Hoi-Ying Elsie Yu, PhD, DABCC, FADLM
Sheng-Ying (Margaret) Lo, PhD, DABCC
Review Date:  05/08/2025

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