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Specimen Collection Manual and Test Catalog

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NRAS MUTATION ANALYSIS, PCR

Geisinger Epic Procedure Code:  LAB2773    Geisinger Epic ID:  45933
Test Restriction:  LAB ORDER ONLY

SPECIMEN COLLECTION
Specimen type:  Formalin-fixed, paraffin-embedded tissue (FFPE)
Preferred collection container: 
Specimen required: 
Two (2) 10 micron sections in 50 mL tube (preferred), four (4) 10 micron FFPE unstained slides with H&E, or paraffin block.
Special notes: 
For requests from non-Geisinger sites, please send: Five (5) 10 micron FFPE sections (scrolls)- preferred specimen, H&E slide, source of specimen, and tumor assessment (<10% or >10%). Alternatively, send FFPE block. Isolated/extracted nucleic acids are acceptable only from CLIA-certified laboratories or a laboratory meeting equivalent requirements as determined by CAP and/or CMS.

SPECIMEN PROCESSING
Transport temperature: 
Room temperature (18-25°C).
Specimen stability: 
Indefinitely.
Rejection criteria: 
Blocks containing no tumor (evaluted by pathologist) will be rejected. FFPE tissues that have been decalcified. Insufficient DNA.

TEST DETAILS
Reference interval: 
Negative for mutation(s) tested.
CPT code(s):  81311
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 
Identification of NRAS mutations in codons 12, 13, 59, 61, 117, and 146.
Methodology: 
Real-time Polymerase Chain Reaction (PCR)
Synonyms: 
NRAS, N-ras, NRAS gene, N-RAS protein
Clinical significance: 
Testing for NRAS mutations is used to predict response to anti-EGFR and MAPK pathway therapies in a variety of malignancies (e.g. colorectal and lung cancer).
Doctoral Director(s): 
Yi Ding MD, PhD
Review Date:  09/24/2024

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