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Specimen Collection Manual and Test Catalog

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VITAMIN B1 (THIAMINE), BLOOD, LC/MS/MS

Geisinger Epic Procedure Code:  LAB3255    Geisinger Epic ID:  47717

SPECIMEN COLLECTION
Specimen type: 

Whole blood


Preferred collection container: 
Alternate collection container: 
3 mL lavender-top (K2 EDTA) tube
Specimen required: 

2 mL EDTA whole blood, protected from light. Minimum 1 mL EDTA whole blood protected from light.


Special notes: 

Protect specimen from light.



SPECIMEN PROCESSING
Processing instructions: 

DO NOT SPIN! Transfer whole blood to amber transport tube. If amber tube is not available, wrap tube in aluminum foil to protect from light. FREEZE IMMEDIATELY.


Transport temperature: 

Frozen.


Specimen stability: 

Room temperature: 8 hours. Refrigerated: 5 days. Frozen: 35 days.


Rejection criteria: 

Specimen not protected from light. Plasma.



TEST DETAILS
CPT code(s):  84425
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Methodology: 
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
Synonyms: 

Quest test code 5042, B1,Thiamine, VB1WB


Clinical significance: 

Vitamin B1 is required for branched-chain amino acid and carbohydrate metabolism. Vitamin B1 deficiency is most often due to alcoholism or chronic illness. In the early stage, patients with vitamin B1 deficiency exhibit anorexia, irritability, apathy, and generalized weakness. Prolonged deficiency causes beriberi.


Doctoral Director(s): 
Randin Nelson MD
Review Date:  04/05/2024

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