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Specimen Collection Manual and Test Catalog

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CORD BLOOD ABO/RH AND DIRECT COOMBS (IGG)

Geisinger Epic Procedure Code:  LAB2078    Geisinger Epic ID:  54320

SPECIMEN COLLECTION
Specimen type: 

Whole blood


Preferred collection container: 
Alternate collection container: 
2 mL pink-top K2 EDTA tube
Specimen required: 

6 mL cord blood collected in lavender-top (EDTA) tube (preferred specimen).


Special notes: 

Specimen must be labeled with patient’s name(e.g., SMITH, GIRL OFMARY) and medical record number. Date of collection and phlebotomist identification (full name or tech ID) must be available. Positive patient identification must be assured. If a cord specimen is unable to be obtained, an ABORh and DAT test must be ordered and a heel stick specimen obtained.



SPECIMEN PROCESSING
Processing instructions: 

Do not centrifuge or aliquot.


Transport temperature: 

Room temperature preferred (less than 24 hours). If greater than 24 hours, specimen should be stored at 2-8°C.


Specimen stability: 

Specimen must be less than 48 hours old for testing.


Rejection criteria: 

Incorrect specimen collection container or incomplete label ID (see specimen collection notes. Stability limits exceeded.



TEST DETAILS
Critical values/courtesy alerts: 
DAT 2+ or stronger on neonates (courtesy alert)
CPT code(s):  86900, 86901, 86880
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Methodology: 
RBC agglutination and/or gel column technology
Synonyms: 

CDEV


Clinical significance: 

The ABO/Rh is necessary to evaluate newborn’s compatibility with maternal blood and therefore gauge the risk of hemolytic disease of the newborn. The Rh is used to determine if the mother is a candidate for Rh Immune Globulin. The DAT determines if red cells have been coated in vivo with immunoglobulins (IgG only) and is used primarily for investigation of hemolytic disease of the fetus and newborn.


Doctoral Director(s): 
Randin Nelson MD
Review Date:  10/06/2024

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