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Specimen Collection Manual and Test Catalog

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BCR-ABL1 P190, MONITOR, RT-QPCR

Geisinger Epic Procedure Code:  LAB3447    Geisinger Epic ID:  60553

SPECIMEN COLLECTION
Specimen type:  Whole blood or bone marrow
Preferred collection container: 
Alternate collection container: 
3 mL lavender-top (K2 EDTA) tube
Specimen required: 
Whole blood: One (1) 6 mL pink-top K2 EDTA tube; minimum 4 mL; (preferred); OR two (2) 3 mL lavender-top K2 EDTA tubes (also acceptable).
Bone marrow: One (1) 3 mL lavender-top K2 EDTA tube (part of Bone Marrow Panel Collection – Bone Marrow Aspirate: Two (2) K2 EDTA tube(s)).
Special notes: 
For molecular testing, original tube is required.

SPECIMEN PROCESSING
Processing instructions: 
Mix well immediately. Send/deliver collection tube to GMC Molecular Diagnostics Laboratory. Store at 2-8°C. Do not centrifuge.
Transport temperature: 
Refrigerated, 2-8°C.
Specimen stability: 
Refrigerated, 2-8°C: 3 days. Specimen must be tested within 3 days. If needed, specimens must be extracted within 3 days for RNA.
Rejection criteria: 
Wrong collection tube. Improper storage or transport temperature. Stability limits exceeded. Shared or comingled specimens.

TEST DETAILS
Reference interval: 
Negative. The BCR-ABL1 190 fusion transcript (e1a2) is not detected.
Additional information: 
The lower limit of detection (LLoD) and lower limit of quantitation (LoQ) for the BCR-ABL1 p190 assay is 0.0100% ratio. Supporting procedural data for this assay lists the LLoD and LoQ as 0.0100% ratio. For molecular testing, original tube(s) required.
CPT code(s):  81207
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 
The results of this test are reported in BCR-ABL1/ABL1% ratio. This test does not detect the rare BCR-ABL1 micro (p230) fusion form. For the p210 fusion form (major breakpoint), order BCR-ABL1 p210, Monitor, RT-qPCR.
Methodology: 
Polymerase Chain Reaction (PCR)
Reverse Transcription Polymerase Chain Reaction (RT-PCR)
Synonyms: 
BCR, ABL, BCR-ABL, BCR-ABL1, p190, BA190M
Clinical significance: 
This test should be used to monitor response to therapy in patients with known e1/a2 BCR-ABL1 p190 fusion forms.
Doctoral Director(s): 
Yi Ding MD, PhD
Review Date:  09/24/2024

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