Specimen Collection Manual and Test Catalog

Three (3) 6 mL pink-top K2 EDTA tubes (minimum 4 mL each) whole blood OR six (6) 3 mL lavender-top K2 EDTA tubes.

Collect three (3) 6 mL pink-top K2 EDTA tubes OR six (6) 3 mL lavender-top K2 EDTA tubes. NOTE: Isolated/extracted nucleic acids are acceptable only from CLIA-certified laboratories or a laboratory meeting equivalent requirements as determined by CAP and/or CMS.

Mix well immediately. Send/deliver all collection tubes to GMC Molecular Diagnostic Lab. Store at 2-8°C. Do not centrifuge.

Refrigerated, 2-8°C. Send to GMC Molecular Diagnostic Lab.

Refrigerated, 2-8°C: 3 days. Specimen must be extracted within 3 days.

Wrong collection tube. Improper storage or transport temperature. Stability limits exceeded. Shared or comingled specimens.

Negative (no mutations detected).

For molecular testing, original tube required.

BCR-ABL1, JAK2, CALR, MPL, and CSF3R mutations detected by Next Generation Sequencing. 

 

RNA Based BCR-ABL1 Analysis: Provides qualitative information on the absence or presence of BCR-ABL1 fusion transcripts including the most common BCR-ABL1 transcripts e1a2 (p190), e13a2 (p210), and e14a2 (p210) at = 0.1% frequency in wild-type background. In addition to the e1a2, e13a2, and e14a2 transcript variants, this assay is predicted to detect rare alternative BCR-ABL1 break/fusion events including e19a2 (p230). False negative results are possible if mutant cells are below the analytical sensitivity of the method or alternative break/fusion events that result in unusual BCR-ABL1 transcript types.                                                                                 

BCRABL, BCR-ABL1, p210, p190, JAK2, JAK-2, Janus Tyrosine Kinase 2, CALR, Calreticulin, MPL, Thrombopoietin Receptor, CSF3R, Colony Stimulating Factor 3 Receptor, Thrombocytosis, MPD, Myeloproliferative Disorder, MPDSP

This test detects mutations associated with myeloproliferative disorders that are informative in the diagnosis and management of disease.