Specimen Collection Manual and Test Catalog

3 mL bone marrow (minimum 1 ml) or 5 mL whole blood ( minimum 3 ml) collected in a sodium heparin (green-top) tube (preferred). Bone marrow collected in transport medium also acceptable.

Do not centrifuge or refrigerate.

Room temperature.

Transport immediately after collection. Viability decreases during transit. Send specimen to testing lab for viability determination. Do not freeze. Do not reject.

Frozen specimens unacceptable.

Quest test code 39035, FISHPH

FISH, Ph Like ALL Panel - Philadelphia chromosome (Ph)-like acute lymphoblastic leukemia (ALL), also referred to as a BCR-ABL1-like ALL, is a high-risk subset with a gene expression profile that shares significant overlap with that of Ph-positive (Ph+) ALL and is suggestive of active kinase signaling. It exibits adverse clinical features, confers poor prognosis and harbors a diverse range of genetic alterations that active cytokine receptors genes and kinase signaling pathways making it amenable to treatment with tyrosine kinase inhibitor (TKI) therapy. This FISH panel has been designed to detect ABL1, ABL2 and PDGFRB rearrangements associated with the BCR-ABL1-like B-ALL (or Ph-like B-ALL) with ABL class fusions. Diagnosis of BCR-ABL1-like B-ALL patients with ABL1, ALB2 and PDGFRB rearrangements, will enable incorporation of TKI much earlier in the course of treatment as well as selection of patients eligible for future therapy trials