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Specimen Collection Manual and Test Catalog

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PROTEIN C ACTIVITY

Geisinger Epic Procedure Code:  LAB2932    Geisinger Epic ID:  17588

SPECIMEN COLLECTION
Specimen type: 

Platelet-poor plasma


Preferred collection container: 
Alternate collection container: 
Other size blue-top (3.2% sodium citrate) tubes (e.g., 1.8 mL, 4.5 mL)
Specimen required: 

1 mL


Special notes: 
  • If the patient’s hematocrit (HCT) is >55%, the volume of anticoagulant in the tube must be adjusted. Contact a performing location for sodium citrate adjusted tubes or instruction for how to adjust the sodium citrate.
  • When possible, use fresh venipuncture
  • Avoid prolong tourniquet time (< 1 minute) and hemolysis during collection as this will alter results/li>
  • If indwelling catheter or butterfly collection device must be used, draw sample from a non-heparinized lumen or flush the line with 5 mL of saline and discard the first 10 mL of blood.
  • Tubes should be >90% filled.
  • Immediately mix gently after collection by inverting the tube end over end 5 to 6 times. Avoid vigorous mixing or additional inversion. Observe for the presence of clots and recollect if observed.

Patient preparation: 

Patient must be off Coumadin 2 weeks prior to testing.



SPECIMEN PROCESSING
Processing instructions: 

Centrifuge as soon as possible (within 60 minutes) at designated speed and time to obtain platelet-poor plasma (< 10,000/uL). Remove plasma and re-spin the plasma for 10 minutes at 3000 rpm and submit platelet free plasma.

For a single NaCit blue top tube collection, create 1 aliquot NaCit and if additional plasma remains, create an additional aliquot NaCit. If multiple tubes collected, create 1 aliquot NaCit for each NaCIt blue top tube collected plus one additional aliquot NaCit. Aliquots should be 1.0-1.9 mL and all plasma-based coagulation tests must be moved to aliquot(s).


Transport temperature: 

Frozen on dry ice


Specimen stability: 

Room temperature (whole blood): 4 hours 
Room temperature (plasma off cells): 8 hours 
Frozen -20°C: 30 days 
Frozen -70°C: 6 months


Rejection criteria: 

Clotted, hemolyzed, improperly filled tubes, improper anticoagulant ratio (HCT >55% and citrate not adjusted), refrigerated specimens, or stability exceeded.

Note: Specimen suspected of thawing in transport, indicated by slant in aliquot or specimen in lid of tube, will have testing performed, and the comment “interpret results with caution as thawing suspected” added to results.



TEST DETAILS
Reference interval: 

75-135%


Additional information: 

Anticoagulation Impact on Coagulation Tests


CPT code(s):  85303
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Protein C activity


Methodology: 
Chromogenic
Synonyms: 

Functional Protein C, PROTC


Clinical significance: 

Protein C is a vitamin K-dependent protein produced by the liver that hydrolyzes factors Va and VIIIa to prevent clot formation. Low protein C levels can be associated with an increase irisk for blood clot. Acquired causes include liver disease, pregnancy, vitamin K deficiency, coumadin therapy, proinflammatory state, and disseminated intravascular coagulopathy (DIC).


Doctoral Director(s): 
Michelle Grant DO
Review Date:  09/03/2026

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