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Specimen Collection Manual and Test Catalog

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MITOGEN- AND ANTIGEN-INDUCED LYMPHOCYTE PROLIFERATION PANEL

Geisinger Epic Procedure Code:  LAB1058    Geisinger Epic ID:  52157

SPECIMEN COLLECTION
Specimen type: 

Whole blood


Preferred collection container: 
Specimen required: 

20 mL whole blood; minimum 10 mL


Special notes: 

Whole blood must be transported at room temperature and delivered to Quest testing laboratory within 48 hours after collection. Maintain and transport blood at room temperature. Avoid temperatures <15° C and >37°C. In hot weather, it may be necessary to pack the specimen in a container with insulating material around it and place this container inside another one that contains a cold pack (ice pack) and absorbent material. This method will help retain the specimen at ambient temperature. For longitudinal studies, draw specimens at the same time of day to minimize diurnal variation.



SPECIMEN PROCESSING
Processing instructions: 

Test available by prior arrangement only. Please contact Referred testing to alert collection. Do not collect later than 1 pm. Collect Monday through Thursday only. No Holiday collections. Sample must be delivered to Referred testing before 1:30 pm. Referred Testing: Please refer to Quest Short Stability Protocol.


Transport temperature: 

Room temperature.


Specimen stability: 

Room temperature: 48 hours. Refrigerated: Unacceptable. Frozen: Unacceptable.


Rejection criteria: 

Hemolysis. Received refrigerated. Received frozen. Clotted specimens. Samples collected in lithium heparin tubes.



TEST DETAILS
CPT code(s):  86353 x6
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Phytohemagglutinin (PHA)-induced Lymphocyte Proliferation; Concanavalin A (Con A)-induced Lymphocyte Proliferation; Pokeweed Mitogen (PWM)-induced Lymphocyte Proliferation; Candida-induced Lymphocyte Proliferation; Tetanus-induced Lymphocyte Proliferation; Tuberculin PPD-induced Lymphocyte Proliferation


Methodology: 
Cell Culture
Scintillation Counter
Synonyms: 

Quest test code 91978, Mitogen Pro, Lymphocyte Stimulation,Antigen-induced Cell Proliferation,Tetanus Toxoid, mitogen/antigen-induced,Mitogen-induced Cell Proliferation,Candida, mitogen/antigen-induced,Tuberculin PPD (TB PPD), mitogen/antigen-induced


Clinical significance: 

Measurement of human lymphocytes' proliferative responses to various stimuli is a fundamental technique used to assess their biological status and functions. Mitogens, such as plant lectins phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), are able to nonspecifically stimulate lymphocyte proliferation and used to evaluate patient immune responsiveness. Lymphocyte proliferation response to antigens, such as Candida, tetanus toxoid and tuberculin purified protein derivative (PPD), are evaluated as a function of memory in cell-mediated immunity.


Review Date:  12/05/2024

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