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Specimen Collection Manual and Test Catalog

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METHYLMALONIC ACID AND HOMOCYSTEINE

Geisinger Epic Procedure Code:  LAB1196    Geisinger Epic ID:  52709

SPECIMEN COLLECTION
Specimen type: 

Serum (preferred) or plasma


Preferred collection container: 
Alternate collection container: 
4 mL green/green-top (sodium heparin) tube
3.5 mL gold-top (serum separator) tube
Specimen required: 

2.7 ml serum (preferred) or plasma: minimum 1.1 mL.



SPECIMEN PROCESSING
Processing instructions: 

Red-top tubes (preferred): Place the specimen in a refrigerator or ice bath for 30 minutes after collection. Centrifuge the specimen as soon as possible after complete clot formation has taken place. Transfer the serum to a transport tube.

Barrier gel separator tubes (acceptable): Place the specimen in a refrigerator for 30 minutes after collection. Centrifuge the specimen as soon as possible after complete clot formation has taken place. Do not place barrier tubes in an ice bath as freezing may prevent the barrier gel from adequately separating serum from cells. 

Heparin tubes (acceptable): Place the specimen in a refrigerator or ice bath until the specimen can be centrifuged. Centrifuge the specimen as soon as possible and transfer the plasma to a transport tube.


Transport temperature: 

Room temperature.


Specimen stability: 

Room temperature: 4 days. Refrigerated: 7 days. Frozen: 6 months.


Rejection criteria: 

Gross hemolysis • Unseparated serum or plasma • Whole blood samples • Grossly lipemic



TEST DETAILS
Additional information: 

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.


CPT code(s):  83090, 83921
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Methodology: 
Enzymatic
Tandem Mass Spectrometry
Synonyms: 

Quest test code 30517 Mma/Homocyst


Clinical significance: 

To diagnose and monitor vitamin B12 (cobalamin) deficiency.


Review Date:  12/09/2024

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