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Specimen Collection Manual and Test Catalog

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CENTROMERE ANTIBODY

Geisinger Epic Procedure Code:  LAB1237    Geisinger Epic ID:  52873

SPECIMEN COLLECTION
Specimen type: 

Serum


Preferred collection container: 
Specimen required: 

0.5 mL serum; minimum 0.2 mL



SPECIMEN PROCESSING
Processing instructions: 

Allow to clot. Centrifuge and submit 0.5 mL serum.


Transport temperature: 

Refrigerated (preferred). Room temperature (stable 8 hours) or frozen specimen also acceptable.


Specimen stability: 

Room temperature: 8 hours. Refrigerated (2-8°C): 2 weeks. Frozen: 6 months.



TEST DETAILS
Reference interval: 

Negative <7 U/mL


Interpretation guide: 

Negative: <7 U/mL 
Equivocal: 7-10 U/mL 
Positive: >10 U/mL


CPT code(s):  86235
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Methodology: 
Enzyme Immunoassay (EIA)
Synonyms: 

ACA, centromere b antibody, CENP, CENP-B


Clinical significance: 

The presence of centromere B antibodies in correlation with clinical findings can aid in the diagnosis of systemic scleroderma, especially the limited cutaneous (CREST syndrome) manifestation. Centromere antibodies are found in 70-90% of patients with CREST syndrome, but may also be found in 10-20% of patients with Raynaud's phenomenon or primary biliary cirrhosis, and may also be positive in patients with SLE.


Doctoral Director(s): 
Benjamin Andress, PhD, DABCC
Hoi-Ying Elsie Yu, PhD, DABCC, FADLM
Review Date:  01/14/2025

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