Specimen Collection Manual and Test Catalog

2 mL aliquot serum; minimum 0.5 mL.

Allow serum to clot. Centrifuge to separate serum within 2 hours of collection.

Refrigerated.

Refrigerated: 3 weeks. Frozen: 3 months.

Stability limits exceeded.

Kappa FLC: 3.30-19.40 mg/L.
Lambda FLC: 5.71-26.30 mg/L
Kappa/Lambda FLC Ratio: 0.26-1.65.

Decreased kidney function can cause an increase in serum Kappa and Lambda Free Light Chains, and Free Light Chain Ratio. For individuals with decreased kidney function, the following reference intervals apply:

Kappa FLC: eGFR 45-59: 7.8-83.6 mg/L 
                    eGFR 30-44: 8.8-103.3 mg/L 
                    eGFR <30: 11.7-265.1 mg/L

Lambda FLC: eGFR 45-59: 7.3-65.1 mg/L
                       eGFR 30-44: 8.2-73.2 mg/L 
                       eGFR <30: 12.6-150.9 mg/L

Kappa/Lambda FLC Ratio: eGFR 45-59: 0.46-2.62 
                                            eGFR 30-44: 0.48-3.38 
                                            eGFR <30: 0.54-3.30

Kappa FLC, Lambda FLC, Kappa/Lambda FLC Ratio.

Kappa Lambda Serum; Free Light chains, KLS

Measurement of the various amounts of the different types of light chain aids in the diagnosis and monitoring of multiple myleoma, lymphocyte neoplasm, Waldenstrom's macroglobulinemia and connective tissues diseases such as systemic lupus erythematosus.