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Specimen Collection Manual and Test Catalog

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HIV-1 AND 2 ANTIGEN/ANTIBODY, EXPOSURE/OB

Geisinger Epic Procedure Code:  LAB2466    Geisinger Epic ID:  29345

SPECIMEN COLLECTION
Specimen type: 

Whole blood


Preferred collection container: 
Specimen required: 

One (1) full 6 mL EDTA tube


Special notes: 

Client Services notifies Microbiology Laboratory if client calls that specimen is en route. Transport specimen immediately to the lab for testing. 
This assay is intended for the detection if HIV-1 p24 antigen and antibodies to HIV-1 and HIV-2 in individuals of unknown HIV status. It is not intended for use in patients with a previously established diagnosis of HIV infection.
This assay has not been evaluated for newborn screening or for the use with cord blood specimens or for use with specimens from individuals less than 12 years of age. 

THIS TEST IS ONLY PERFORMED FOR SPECIAL CIRCUMSTANCES. PLEASE SEE "ADDITIONAL INFORMATION!"



SPECIMEN PROCESSING
Processing instructions: 

Do not centrifuge. Do not aliquot.


Transport temperature: 

Room temperature (15-30°C) for <48 hours or refrigerated (2-8°C).


Specimen stability: 

Stable for up to 48 hours at room temperature (15-30°C). Stable 6 days at refrigerated (2-8°C).


Rejection criteria: 

Improperly labeled/identified specimens. Specimens stored or transported beyond stability limits. Expired transport device used. Improper collection tube used. Specimen received frozen.



TEST DETAILS
Reference interval: 

Negative for HIV 1 and/or 2 antibodies, Negative for HIV 1 p24 antigen.


Critical values/courtesy alerts: 
Positive for HIV 1 and/or 2 antibodies, Positive for HIV 1 p24 antigen.
Additional information: 

THIS TEST IS ONLY PERFORMED FOR EMPLOYEE HEALTH SERVICE, SELECTED OUTREACH CLIENTS, OBGYN PATIENTS AT DELIVERY WHEN THE HIV STATUS IS NOT KNOWN AND FOR OTHER PATIENTS WHEN APPROVED BY THE MICROBIOLOGY DOCTORAL DIRECTOR, PATHOLOGIST ON CALL OR ANALYTICAL SPECIALIST.

Interfering Substances:

  1. Specimens from individuals infected with HIV-1 and/or HIV-2 who are receiving antiretroviral (ART) therapy may produce false negative test results
  2. Specimens from individuals with Toxoplasma IgG, human anti-mouse antibodies, rheumatoid factor, elevated triglycerides (above 600 mg/dL), herpes simplex virus infection, hospitalized and cancer patients may give false positive test results.

CPT code(s):  87806
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Qualitative immunoassay to detect antibodies to Human Immunodeficiency Virus (HIV) 1 and/or 2. Test progresses to an HIV confirmation (HIVCC) at an additional charge if the results of this assay are positive. This confirmatory testing is required and performed at GMC.


Synonyms: 

RPDHIV


Clinical significance: 

Use this test to detect antibody to HIV 1 and/or HIV 2 and HIV 1 p24 antigen. HIV 2 is endemic in West Africa and most cases in the U.S. are from West Africa or people who have traveled in that area. Not intended for use in individuals with a known HIV-positive status. Used for baseline and follow-up testing after bloodborne pathogen exposures by employee health. Utilized for prenatal HIV screening in accordance with current screening recommendations.


Doctoral Director(s): 
Donna Wolk PhD, D(ABMM)
Julie Hirschhorn, PhD, HCLD(ABB)
Review Date:  09/22/2026

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