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Specimen Collection Manual and Test Catalog

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CELL COUNT WITH DIFFERENTIAL, BODY FLUID

Geisinger Epic Procedure Code:  LAB1971    Geisinger Epic ID:  7683

SPECIMEN COLLECTION
Specimen type: 

Pericardial fluid, peritoneal (ascitic) fluid, pleural, amniotic fluid, and other serous fluids (non-synovial)


Preferred collection container: 
Alternate collection container: 
Sterile screw-cap tube
6 mL red-top (plain, non-serum separator) tube
4 mL green/green-top (sodium heparin) tube
Specimen required: 

2-3 mL (preferred), 1 mL (minimum)


Special notes: 

Indicate specimen source on label and requisition. Clotted and partially clotted specimens will receive a comment, "Fluid clotted cell count questionable". Fluids received in sodium heparin tubes will receive a comment, "Specimen received in heparin anticoagulant which may cause lower WBC counts."



SPECIMEN PROCESSING
Processing instructions: 

Do not centrifuge or freeze.


Transport temperature: 

Refrigerated.


Specimen stability: 

24 hours.


Rejection criteria: 

BAL specimens, gastric specimens, and semen analysis specimens. Specimen sent with needle. Frozen specimens. Clotted specimens.



TEST DETAILS
Reference interval: 

Varies based on specimen type.


Interpretation guide: 

Red cells are increased in trauma and hemorrhage. 
White blood cells are increased in inflammatory and infectious processes: 
- neutrophils predominate in bacterial infections
- lymphocytes predominate in viral infection
- macrophages may be increased in inflammatory and infectious processes
- eosinophils may be increased in parasitic or fungal infections.


CPT code(s):  89051
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Test includes: 

Color, Clarity, Total nucleated cell count (TNC) (Note: This includes WBC count and other nucleated cells including epithelial cells, histiocytes, macrophages, mesothelial cells etc.) , RBC, TNC differential = % of each cell type in 100 total nucleated cells.


Methodology: 
Electronic Particle Cell Counter
Electronic resistance detection enhanced by hydrodynamic focusing and flow cytometry
Hemocytometer
Microscopic Examination
Synonyms: 

BFCD, BFC, cell count FLD


Clinical significance: 

Quantitative determination of cells including WBCs, RBCs, and lining cells in various body fluids (excluding bronchoalveolar lavage specimens), which aids in the diagnoses of systemic disease, inflammation, malignancy, infection, and trauma.


Doctoral Director(s): 
Mary Dhesi MD
Review Date:  01/08/2025

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