Specimen Collection Manual and Test Catalog

1 mL aliquot of plasma for each time interval; minimum 0.2 mL.

Specimens must be collected on a time schedule (Fasting, 0.5 hour, 1 hour, 1.5 hour, 2 hour, 3 hour, 4 hour and 5 hour). Place specimen on ice, or centrifuge and separate plasma from cells.

8-hour fasting is required. After collection of fasting specimen, 75 g of glucose should be given immediately for adult. For children (under 18 years of age), 1.75 g glucose/kg ideal body weight up to a maximum dose of 75 g or per physician request.

Centrifuge to separate plasma within 2 hours of collection.

Refrigerated (preferred).

3 days (spun and unspun).

Stability limits exceeded.

Fasting: 70-99 mg/dL
0.5 hour post challenge: 70-170 mg/dL
1 hour post challenge: 70-170 mg/dL
1.5 hour post challenge: 70-170 mg/dL
2 hr post challenge: 90-139 mg/dL
3 hr post challenge: 70-120 mg/dL
4 hr post challenge: 70-120 mg/dL
5 hr post challenge: 70-120 mg/dL

Fasting glucose (GSFAST), 1/2-hour glucose (GS05), 1-hour glucose (GS10), 1-1/2 hour glucose (GS15), 2-hour glucose (GS20), 3-hour glucose (GS30), 4-hour glucose (GS40), 5-hour glucose (GS50)

This test can be used to evaluate hypoglycemia.