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Specimen Collection Manual and Test Catalog

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METHOTREXATE LEVEL

Geisinger Epic Procedure Code:  LAB2674    Geisinger Epic ID:  14268

SPECIMEN COLLECTION
Specimen type: 

Serum


Preferred collection container: 
Specimen required: 

2 mL serum; minimum 1 mL.


Special notes: 

Protect from light recommended.



SPECIMEN PROCESSING
Processing instructions: 

Allow serum to clot. Centrifuge to separate serum within 2 hours of collection.


Transport temperature: 

Refrigerated (preferred). Room temperature or frozen specimen also acceptable.


Specimen stability: 

Room temperature: 2 days. Refrigerated: 7 days. Frozen: 30 days.


Rejection criteria: 

Stability limits exceeded.



TEST DETAILS
Reference interval: 

Not specified.


CPT code(s):  80204
Note: The billing party has sole responsibility for CPT coding.  Any questions regarding coding should be directed to the payer being billed.
The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only.

Methodology: 
Homogeneous enzyme immunoassay
Synonyms: 

METHOA , MTX


Clinical significance: 

Used to quantitatively measure methotrexate in serum. Methotrexate is an antineoplastic drug used in the treatment of leukemia and other diseases.


Doctoral Director(s): 
Hoi-Ying Elsie Yu, PhD, DABCC, FADLM
Sheng-Ying (Margaret) Lo, PhD, DABCC
Review Date:  12/17/2024

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