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Geisinger Medical Laboratories Test Catalog
DIRECT COOMBS |
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Geisinger Epic Procedure Code: LAB2189
Geisinger Epic ID: 20200
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SPECIMEN COLLECTION |
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Specimen type: |
Whole Blood | |
Preferred collection container: |
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Alternate Collection Container: |
Lavender-top (K2 EDTA) microcollection tube
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Specimen required: |
6 mL whole blood; minimum 1 mL. | |
Special notes: |
Specimen must be labeled with patient's first and last name, medical record number or some unique other identifier (e.g.,billing number, NOT date of birth). Date of collection and phlebotomist identification (full name or tech ID) must be available. Positive patient identification must be assured. For cord blood samples, order CDEV (Cord Evaluation) test battery for ABORH and DAT testing. | |
| SPECIMEN PROCESSING |
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Specimen processing instructions: |
Do not centrifuge or aliquot. | |
Transport temperature: |
Room temperature preferred (less than 24 hours). If greater than 24 hours, specimen should be stored at 2-8°C. | |
Specimen stability: |
Specimen must be less than 48 hours old for testing. | |
Rejection criteria: |
Tube containing gel separator. Incomplete label ID (see specimen collection notes). | |
TEST DETAILS |
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Critical values/courtesy alerts: |
DAT 2+ or stronger on neonates (courtesy alert). | |
CPT code(s): |
86880 | |
| Note: The billing party has sole responsibility for CPT coding. Any questions regarding coding should be directed to the payer being billed. The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only. | ||
Test includes: |
DAT | |
Methodology: |
RBC agglutination and/or gel column technology |
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Synonyms: |
Direct Coombs, DAT
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Clinical significance: |
DAT determines if red cells have been coated in vivo with immunoglobulins (IgG only), complement (C3 only) or both. Primarily for investigation of hemolytic transfusion reaction, hemolytic disease of the fetus and newborn, autoimmune hemolytic anemia and drug-induced immune hemolysis. | |
Doctoral Director(s:) |
Randin Nelson MD | |
| Review Date: | 10/21/2022 | |
Performing Locations
Geisinger Wyoming Valley Medical Center