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Geisinger Medical Laboratories Test Catalog
VITAMIN B7 (BIOTIN) |
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Geisinger Epic Procedure Code: LAB1201
Geisinger Epic ID: 52729
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SPECIMEN COLLECTION |
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Specimen type: |
Serum | |
Preferred collection container: |
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Alternate Collection Container: |
6 mL red-top (plain, non-serum separator) tube
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Specimen required: |
2 mL frozen serum protected from light; minimum 1 mL | |
Special notes: |
Protect from light. | |
| SPECIMEN PROCESSING |
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Specimen processing instructions: |
Allow sample to clot for 30 minutes. Centrifuge at 3000 rpm for 10 minutes and pour serum into a transport tube. Wrap in foil to protect from light. Freeze immediately. | |
Transport temperature: |
Frozen. | |
Specimen stability: |
Room temperature: Unacceptable. Refrigerated: 7 days. Frozen: 14 days. | |
Rejection criteria: |
Hemolyzed specimens • Lipemic specimens • Ambient temperature specimens • Not light protected • Specimens outside of listed stability | |
TEST DETAILS |
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Additional information: |
The performance characteristics of the listed assay was validated by BioAgilytix Diagnostics. The US FDA has not approved or cleared this test. The results of this assay can be used for clinical diagnosis without FDA approval. BioAgilytix Diagnostics is a CLIA certified, CAP accredited laboratory for performing high complexity assays such as this one. | |
CPT code(s): |
84591 | |
| Note: The billing party has sole responsibility for CPT coding. Any questions regarding coding should be directed to the payer being billed. The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only. | ||
Methodology: |
Bioassay |
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Synonyms: |
Quest test code 391, Vitamin B7
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Clinical significance: |
Biotin, vitamin B7, or vitamin H, is a water soluble vitamin. The vitamin plays a role in the transferring of carbon dioxide in the metabolism of fat, carbohydrate and protein by functioning as an enzyme cofactor. It is involved in multiple biochemical reactions including niacin metabolism, amino acid degradation, and the formation of purine, which is an integral part of nucleic acids. It interacts with histone by the action of biotinyl-transferase. Sometimes the vitamin is used in weight reduction programs. It may be prescribed as a supplement for diabetic patients due to its role in carbohydrate metabolism. Biotin is commonly found in vitamin B complex and many food sources, such as milk, yeast, egg yolk, cereal, and mushrooms. The reference daily intake [RDI of 101.9(c) (8) (IV)] for vitamin B7 is 300 micrograms. Deficiency in the vitamin may result in seborrheic dermatitis, alopecia, myalgia, hyperesthesia, and conjunctivitis. Disorders of biotin metabolism can be acquired or congenital. Biotinidase and holocarboxylase synthethase deficiency are the two better known forms of disorders. The lack of biotin-dependent pyruvate carboxylase, propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, and acetyl-CoA carboxylase can lead to the life-threatening disorder of multiple carboxylase deficiency. Treatment involves a daily dose of approximately 10 mg biotin/day. Irreversible mental or neurological abnormalities may result from delayed clinical intervention. | |