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Geisinger Medical Laboratories Test Catalog
HEPATITIS B SURFACE ANTIGEN WITH REFLEX TO PCR
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Geisinger Epic Procedure Code: LAB4792
Geisinger Epic ID: 183960
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SPECIMEN COLLECTION |
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Specimen type: |
Serum AND plasma.
Collect two tubes |
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Preferred collection container: |
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Specimen required: |
1 mL aliquot, minimum 0.5 mL SST tube and collect one 6 mL pink top EDTA or three 3mL lavender top EDTA tubes | |
Special notes: |
This test is restricted and only orderable by Nephrology/Dialysis. | |
| SPECIMEN PROCESSING |
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Specimen processing instructions: |
Send unspun collection tube to testing lab. Allow serum to clot. Centrifuge to separate serum within 2 hours of collection. Plasma will be separated at testing lab. Separate plasma within one day of collection and transfer plasma to a screw-capped, plastic aliquot tube. Freeze immediately at -20°C. | |
Transport temperature: |
Refrigerated (2-8°C) | |
Specimen stability: |
Serum: Stable 14 days at 2-8°, 6 months frozen. Whole Blood: Refrigerated (2-8°C). Room temperature (15-30°C) up to 24 hours. Plasma: Refrigerated (2-8°C) up to 72 hours. Room temperature (15-30°C) up to 20 hours. Frozen (-20°C) up to 60 days. Can be subjected to at most 3 freeze thaw cycles. Thawed samples may be stored at 2-8°C for up to 6 hours prior to testing. Frozen (-70°C) long term. Can be subjected to at most 3 freeze thaw cycles. Thawed samples may be stored at 2-8°C for up to 6 hours prior to testing. |
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Rejection criteria: |
Specimens stored or transported at incorrect temperature, stability limits exceeded, improper collection tube used, insufficient volume, improperly labeled/ identified specimens. | |
TEST DETAILS |
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Reference interval: |
Negative. HBV DNA not detected. | |
Additional information: |
Department of Health is notified for PA residents if positive. | |
CPT code(s): |
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| Note: The billing party has sole responsibility for CPT coding. Any questions regarding coding should be directed to the payer being billed. The CPT codes provided by GML are based on AMA guidelines and are for informational purposes only. | ||
Test includes: |
Qualitative detection of hepatitis B surface antigen and 1uantitation of hepatitis B viral DNA by PCR. | |
Methodology: |
Electrochemiluminescence Immunoassay |
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Synonyms: |
HBSAG, Hepatitis Associated Antigen Hepatitis B surface antigen Hepatitis B Viral Load, HBVL, Viral Load, Hepatitis B, Hep B, Hep B Viral Load, Quant Viral Load
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Clinical significance: |
This test is utilized for the detection and quantitation of hepatitis B virus (HBV) DNA in the plasma of patients with HBV infection (i.e., hepatitis B surface antigen positive), monitoring disease progression in chronic HBV infection, and monitoring response to anti-HBV therapy. | |
Doctoral Director(s:) |
Hoi-Ying Elsie Yu, PhD, DABCC, FADLM / Donna Wolk PhD, D(ABMM) | |
| Review Date: | 10/25/2023 | |